Related Experiment Video
Updated: Jan 9, 2026
Immunopeptidomics: Isolation of Mouse and Human MHC Class I- and II-Associated Peptides for Mass Spectrometry Analysis
Published on: October 15, 2021
Methods for human CD8(+) T lymphocyte proteome analysis
Lynne Thadikkaran1, Nathalie Rufer, Corinne Benay
1Service Régional Vaudois de Transfusion Sanguine, Lausanne, Switzerland.
T lymphocytes, including cytotoxic CD8(+) T cells, are important cells involved in immunology, as they can destroy infected or tumor cells. We describe here a detailed protocol starting from CD8(+) T lymphocytes isolation for T cell culture followed by total protein extraction or subcellular fractionation, like nuclei isolation. We also describe well-defined biochemistry and cell biology methods adapted to T lymphocytes, showing the importance of using the method best suited to answering the question addressed. All these techniques would be very helpful to immunologists willing to study underlying biological processes related to T lymphocytes.
T lymphocytes, including cytotoxic CD8(+) T cells, are important cells involved in immunology, as they can destroy infected or tumor cells. We describe here a detailed protocol starting from CD8(+) T lymphocytes isolation for T cell culture followed by total protein extraction or subcellular fractionation, like nuclei isolation. We also describe well-defined biochemistry and cell biology methods adapted to T lymphocytes, showing the importance of using the method best suited to answering the question addressed. All these techniques would be very helpful to immunologists willing to study underlying biological processes related to T lymphocytes.
