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Related Concept Videos

Proteomics01:33

Proteomics

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A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
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Related Experiment Video

Updated: Jan 9, 2026

Immunopeptidomics: Isolation of Mouse and Human MHC Class I- and II-Associated Peptides for Mass Spectrometry Analysis
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Immunopeptidomics: Isolation of Mouse and Human MHC Class I- and II-Associated Peptides for Mass Spectrometry Analysis

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Methods for human CD8(+) T lymphocyte proteome analysis.

Lynne Thadikkaran1, Nathalie Rufer, Corinne Benay

  • 1Service Régional Vaudois de Transfusion Sanguine, Lausanne, Switzerland.

Methods in Molecular Biology (Clifton, N.J.)
|July 2, 2008
PubMed
Summary
This summary is machine-generated.

This study details methods for isolating cytotoxic CD8(+) T lymphocytes and performing protein extraction or subcellular fractionation. These techniques aid immunologists in studying T lymphocyte biology and functions.

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TMT Sample Preparation for Proteomics Facility Submission and Subsequent Data Analysis
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Area of Science:

  • Immunology
  • Cell Biology
  • Biochemistry

Background:

  • T lymphocytes, particularly cytotoxic CD8(+) T cells, are crucial immune cells.
  • These cells play a vital role in eliminating infected cells and tumor cells.
  • Understanding their function requires robust laboratory techniques.

Purpose of the Study:

  • To provide a detailed protocol for T lymphocyte isolation and subsequent analysis.
  • To describe methods for total protein extraction and subcellular fractionation in T lymphocytes.
  • To highlight the importance of selecting appropriate methods for specific research questions in T cell biology.

Main Methods:

  • Isolation of CD8(+) T lymphocytes from samples.
  • Culturing of isolated T lymphocytes.
  • Total protein extraction from T cells.
  • Subcellular fractionation, including nuclei isolation.
  • Application of biochemistry and cell biology techniques tailored for T lymphocytes.

Main Results:

  • A comprehensive protocol for T lymphocyte manipulation is presented.
  • Established methods for protein extraction and subcellular fractionation are detailed.
  • The study emphasizes the critical choice of methodology for accurate biological insights.

Conclusions:

  • The described techniques are valuable resources for immunologists.
  • These methods facilitate the study of underlying biological processes in T lymphocytes.
  • Proper application of these techniques enhances the investigation of T cell-mediated immunity.