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Related Concept Videos

Flow Cytometry01:23

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Related Experiment Video

Updated: Jul 4, 2026

Techniques for the Analysis of Extracellular Vesicles Using Flow Cytometry
09:39

Techniques for the Analysis of Extracellular Vesicles Using Flow Cytometry

Published on: March 17, 2015

Flow cytometric analysis of cell membrane microparticles.

Monique P Gelderman1, Jan Simak

  • 1Laboratory of Cellular Hematology, CBER FDA, Rockville, MD, USA.

Methods in Molecular Biology (Clifton, N.J.)
|July 2, 2008
PubMed
Summary

Cell membrane microparticles (MPs), released during cell activation or apoptosis, can indicate cellular changes. This study details a flow cytometry assay for analyzing MPs in plasma, aiding in diagnosing vascular diseases.

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Area of Science:

  • Cellular Biology
  • Immunology
  • Pathophysiology

Background:

  • Cell membrane microparticles (MPs) are vesicles shed from cell membranes during activation or apoptosis.
  • Increased MP levels signify cellular alterations and are linked to vascular pathologies.
  • MPs express surface proteins indicating cellular origin and activation status.

Purpose of the Study:

  • To present a three-color flow cytometric assay for immunophenotyping MPs in plasma.
  • To enable the study of circulating endothelial MPs in various diseases.

Main Methods:

  • Development of a three-color flow cytometry assay.
  • Immunophenotyping of MPs in human plasma.
  • Application of the assay to study endothelial MPs in hematological and vascular diseases.

Main Results:

  • The assay allows for detailed immunophenotyping of MPs in plasma.
  • Elevated counts of specific circulating endothelial MP phenotypes were observed in diseases.
  • The assay is adaptable for analyzing MPs in blood products and cell cultures.

Conclusions:

  • The developed flow cytometry assay is effective for analyzing circulating MPs.
  • This method aids in understanding the role of MPs in vascular pathologies.
  • The assay provides a valuable tool for research and clinical diagnostics involving MPs.