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Related Concept Videos

Bioreactor Controls-II01:18

Bioreactor Controls-II

In aerobic fermentations, oxygen is vital for microbial growth and metabolite production. Since air comprises only about 20% oxygen and the gas is poorly soluble in water—just 9 ppm at 20°C—supplying sufficient oxygen becomes a critical challenge, especially in high-demand processes like yeast growth or citric acid production. Even a fully saturated broth may offer only a few seconds of oxygen availability.To address this, sterile or scrubbed air is introduced into the fermentor via a sparger...
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The scale-up of microbial fermentation processes is essential in industrial biotechnology, allowing the transition from laboratory-scale experiments to commercial-scale production while aiming to maintain product yield and quality. This process requires meticulous adjustment of equipment design, process parameters, and contamination control strategies to accommodate increasing culture volumes.At the laboratory scale, cultures are typically maintained in 1 to 10-liter glass or autoclavable...

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Related Experiment Video

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Combining Fluidic Devices with Microscopy and Flow Cytometry to Study Microbial Transport in Porous Media Across Spatial Scales
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Increased mass transfer to microorganisms with fluid motion.

B E Logan1, J W Dettmer

  • 1Environmental Engineering Program, Department of Civil Engineering, University of Arizona, Tucson, Arizona 85721, USA.

Biotechnology and Bioengineering
|May 1, 1990
PubMed
Summary

Fluid flow significantly enhances bacterial nutrient uptake in wastewater treatment bioreactors. Advective motion increases substrate molecule collisions with cells, boosting leucine uptake by up to 65%.

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Published on: May 28, 2007

Area of Science:

  • Environmental microbiology
  • Biochemical engineering
  • Wastewater treatment technologies

Background:

  • Bacterial nutrient uptake is crucial for microbial activity in bioreactors.
  • Understanding the impact of fluid dynamics on microbial processes is essential for optimizing wastewater treatment.
  • Zoogloea ramigera is a key bacterium in wastewater treatment systems.

Purpose of the Study:

  • To investigate the effect of fluid flow and laminar shear on bacterial uptake.
  • To quantify the influence of advective motion on leucine uptake by Zoogloea ramigera.
  • To correlate microbial uptake rates with mass transfer principles.

Main Methods:

  • Examining bacterial uptake under simulated wastewater bioreactor fluid conditions.
  • Measuring leucine uptake by suspended and fixed Zoogloea ramigera cells.
  • Applying Sherwood number correlations to analyze mass transfer rates.

Main Results:

  • Laminar shear rates below 50 s(-1) did not affect leucine uptake in suspended cultures.
  • Leucine uptake increased by 55-65% in cells exposed to a flow field of approximately 1 mm s(-1).
  • Enhanced uptake is consistent with increased substrate molecule-cell collisions due to boundary layer compression.

Conclusions:

  • Advective fluid flow significantly enhances bacterial nutrient uptake in wastewater treatment bioreactors.
  • Mass transfer principles, specifically boundary layer compression, explain the increased uptake.
  • Leucine transport proteins may occupy a small fraction (approx. 1%) of the cell surface area.