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Related Experiment Video

Updated: Jul 4, 2026

Isolation and Identification of Mesenchymal Stem Cells Derived from Adipose Tissue of Sprague Dawley Rats
10:50

Isolation and Identification of Mesenchymal Stem Cells Derived from Adipose Tissue of Sprague Dawley Rats

Published on: April 7, 2023

Adipose-derived stem cells: isolation, expansion and differentiation.

Bruce A Bunnell1, Mette Flaat, Christine Gagliardi

  • 1Division of Gene Therapy, Tulane National Primate Research Center, Tulane University Health Sciences Center, Covington, LA 70433, USA. bbunnell@tulane.edu

Methods (San Diego, Calif.)
|July 3, 2008
PubMed
Summary

Adipose-derived stem cells (ASCs) offer a plentiful source for regenerative medicine. This study details methods for isolating, expanding, and differentiating these multipotent stem cells for tissue engineering applications.

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Last Updated: Jul 4, 2026

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Isolation, Expansion, and Adipogenic Induction of CD34+CD31+ Endothelial Cells from Human Omental and Subcutaneous Adipose Tissue

Published on: July 17, 2018

Area of Science:

  • Regenerative Medicine
  • Stem Cell Biology
  • Tissue Engineering

Background:

  • Regenerative medicine requires reliable stem cell sources.
  • Adipose tissue is an abundant and accessible source of adult stem cells.
  • Adipose-derived stem cells (ASCs) possess multipotent properties suitable for tissue engineering.

Purpose of the Study:

  • To present detailed methods for ASC isolation, expansion, and differentiation.
  • To highlight the utility of ASCs in regenerative medical applications.
  • To provide a protocol applicable across species with minor modifications.

Main Methods:

  • Isolation of stem cells from human adipose tissue.
  • In vitro expansion of adipose-derived stem cells.
  • Induction of differentiation in ASCs.

Main Results:

  • Established protocols for obtaining and culturing ASCs.
  • Demonstrated the multipotent differentiation capacity of ASCs.
  • Validated the applicability of the isolation procedure for various species.

Conclusions:

  • Adipose tissue is a valuable source for stem cells in regenerative medicine.
  • Detailed methods facilitate the use of ASCs in tissue engineering.
  • ASCs are a promising tool for advancing regenerative therapies.