Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Phase Contrast and Differential Interference Contrast Microscopy

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

3D Image Acquisition and Display: Technology, Perception and Applications 2025: introduction.

Applied optics·2026

Same author

Optimized simultaneous generation of dual-wavelength phase and polarization singularities.

Scientific reports·2026

Same author

Feature issue introduction: 3D image acquisition and display: technology, perception and applications.

Optics express·2026

Same author

A group-housing system for an artificial shark uterus.

MethodsX·2026

Same author

Single-pixel microscopy with enhanced lateral resolution.

Optics express·2025

Same author

Red-shifted excitation enhances the sensitivity of red genetically encoded Ca<sup>2+</sup> indicator and enables crosstalk-free two-photon holographic optophysiology.

Journal of biomedical optics·2025

Same journal

Multifunctional reconfigurable terahertz metasurface based on vanadium dioxide phase transition: achieving broadband absorption and efficient polarization conversion.

Applied optics·2026

Same journal

High-Q-factor electromagnetically induced transparency utilizing quasi-bound states in the continuum in an all-dielectric terahertz metasurface.

Applied optics·2026

Same journal

Automated stitching interferometry for high-precision metrology of X-ray mirrors.

Applied optics·2026

Same journal

Experimental demonstration of an approach to designing a metal-dielectric DBR resonant cavity structure.

Applied optics·2026

Same journal

High-precision wavefront reconstruction from a single-shot interferogram using a physics-driven hybrid feature calibration network.

Applied optics·2026

Same journal

Ultra-high-Q Fano resonance based on coupled topological corner states in Kagome photonic crystals.

Applied optics·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jul 4, 2026

Transmission of Multiple Signals through an Optical Fiber Using Wavefront Shaping

Transmission of Multiple Signals through an Optical Fiber Using Wavefront Shaping

Published on: March 20, 2017

Parallel two-step phase-shifting digital holography.

Yasuhiro Awatsuji¹, Tatsuki Tahara, Atsushi Kaneko

¹Division of Electronics, Kyoto Institute of Technology, Matsugasaki, Kyoto, Japan. awatsuji@kit.ac.jp

|July 3, 2008

Summary

This summary is machine-generated.

This study introduces a parallel two-step phase-shifting digital holography method for instant 3D measurements of moving objects. The novel technique simplifies optical setup and enhances measurement accuracy compared to conventional methods.

More Related Videos

Shaping the Amplitude and Phase of Laser Beams by Using a Phase-only Spatial Light Modulator

Shaping the Amplitude and Phase of Laser Beams by Using a Phase-only Spatial Light Modulator

Published on: January 28, 2019

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

Published on: July 5, 2016

Related Experiment Videos

Last Updated: Jul 4, 2026

Transmission of Multiple Signals through an Optical Fiber Using Wavefront Shaping

Transmission of Multiple Signals through an Optical Fiber Using Wavefront Shaping

Published on: March 20, 2017

Shaping the Amplitude and Phase of Laser Beams by Using a Phase-only Spatial Light Modulator

Shaping the Amplitude and Phase of Laser Beams by Using a Phase-only Spatial Light Modulator

Published on: January 28, 2019

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

Published on: July 5, 2016

Area of Science:

Optical Metrology
Digital Holography
Interferometry

Background:

Phase-shifting digital holography (PSDH) is crucial for 3D object measurement.
Sequential PSDH methods are time-consuming, limiting applications for dynamic objects.
Existing parallel PSDH techniques often involve complex optical setups.

Purpose of the Study:

To develop a parallel two-step phase-shifting digital holography (PSDH) technique.
To enable instantaneous 3D measurement of dynamically moving objects.
To simplify the optical configuration for parallel PSDH systems.

Main Methods:

Implementation of a parallel two-step phase-shifting method.
Optical setup utilizing a phase-shifting array device in the reference beam.
Numerical simulation and preliminary experimental validation.

Main Results:

The proposed technique achieves instantaneous 3D measurement.
Numerical and experimental results closely match sequential PSDH.
Performance surpasses conventional digital holography using Fresnel transform alone.

Conclusions:

The parallel two-step PSDH technique is effective for dynamic 3D object measurement.
The simplified optical system is advantageous for practical implementation.
The method offers improved accuracy and speed over conventional approaches.