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Inhibition of Trichoderma reesei cellulase by sugars and solvents
M Holtzapple1, M Cognata, Y Shu
1Department of Chemical Engineering, Texas A&M University, College Station, Texas 77843, USA.
Abstract:
Inhibition of Trichoderma reesei cellulase by sugars (glucose, delta-gluconolactone, and cellobiose) and solvents (ethanol, butanol, and acetone) was studied using cellulose azure. Glucose, cellobiose, ethanol, and butanol were noncompetitive inhibitors, delta-gluconolactone was a mixed inhibitor, and acetone was a noncompetitive activator. Converting cellobiose to glucose reduces the effective inhibitor binding constant by 6 times and converting cellobiose to ethanol reduces it by 16 times.
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