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Updated: Jul 4, 2026

Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control
Published on: March 30, 2015
[Real-time polymerase chain reaction (RT-PCR)]
Katarzyna Wiedro1, Ewa Stachowska, Dariusz Chlubek
1Zakład Biochemii Katedry Biochemii i Chemii Medycznej Pomorskiej Akademii Medycznej w Szczecinie, Szczecin.
Abstract:
The work present PCR (Polymerase Chain Reaction) with the real time analysis of the increase of product allowing quick detection, duplication and identification of even slight amounts of nucleic acids. Essential methods used in molecular diagnostics, based on marked probes and fluorescence phenomenon were discussed. Increased sensitivity of technology, the possibility of analysis and visualization of products during the time of reaction, enabled the progress of highly effective diagnostics applications.
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