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Updated: Jul 4, 2026

Transmitochondrial Cybrid Generation Using Cancer Cell Lines
Published on: March 17, 2023
Mitochondrial DNA from colorectal cancer cells promotes the malignant phenotype of NIH3T3 cells
Yan Li1, Song Weibing, Hong Liu
1Department of Digestive Diseases, Nanfang Hospital, Southern Medical University, 692 Sheng Li Southern Road, Guangzhou, Guangdong Province 510515, PR China.
Abstract:
We investigated the potential role of mitochondrial DNA (mtDNA) in colorectal carcinogenesis by constructing a eukaryotic expression vector of the mitochondrial D-loop gene from colorectal cancer cell SW480 and transfected NIH3T3 cells. The NIH3T3/SW480 cells exhibited a significantly increased growth rate and colony formation rate, and also had a decreased apoptotic rate. Polyploidy and aberrant chromosomes were detected in the NIH3T3/SW480 cells by chromosome karyotype analysis. Our results suggested that mtDNA from colorectal cancer cells promotes the malignant phenotype of NIH3T3 cells. Further study of the biological functions of NIH3T3/SW480 cells might be helpful in understanding the role of mtDNA in colorectal carcinogenesis.
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