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Updated: Jul 4, 2026

Isolation of Fidelity Variants of RNA Viruses and Characterization of Virus Mutation Frequency
Published on: June 16, 2011
The enhanced DNA replication fidelity of a mutant herpes simplex virus type 1 DNA polymerase is mediated by an
Wang Tian1, Ying T Hwang, Charles B C Hwang
1Department of Microbiology and Immunology, State University of New York, Upstate Medical University, Syracuse, New York 13210, USA.
Abstract:
We previously demonstrated that a recombinant herpes simplex virus containing a mutation within the finger domain of DNA polymerase replicated DNA with increased fidelity. In this study, we demonstrate that, compared with wild-type polymerase, the mutant enzyme exhibited improved nucleotide selectivity and a reduced ability to extend from mismatched primer termini, which would contribute to the increased DNA replication fidelity.
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