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Confocal Fluorescence Microscopy

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

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Related Experiment Video

Updated: Jul 4, 2026

Fluorescence detection methods for microfluidic droplet platforms

Fluorescence detection methods for microfluidic droplet platforms

Published on: December 10, 2011

Acoustically levitated droplets: a contactless sampling method for fluorescence studies.

Jork Leiterer¹, Markus Grabolle, Knut Rurack

¹Federal Institute for Materials Research and Testing (BAM), Richard-Willstätter-Str. 11, 12489 Berlin, Germany. jork.leiterer@bam.de

Annals of the New York Academy of Sciences

|July 4, 2008

Summary

This summary is machine-generated.

Acoustic levitation enables container-free fluorescence spectroscopy, overcoming limitations of traditional methods. This technique allows high-concentration measurements and study of distance-dependent fluorescence in nanomaterials.

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Area of Science:

Analytical Chemistry
Spectroscopy
Materials Science

Background:

Traditional fluorescence spectroscopy is limited by sample containers and small sample volumes.
Inner-filter effects hinder measurements at high concentrations in cuvette-based systems.
Sample support materials can interfere with accurate signal acquisition and alter sample properties.

Purpose of the Study:

To introduce acoustic levitation as a novel tool for fluorescence spectroscopy.
To demonstrate the advantages of acoustic levitation for studying concentration-dependent processes.
To explore distance-dependent fluorescence modulations in semiconductor nanocrystals.

Main Methods:

Utilizing acoustic levitation to suspend small liquid and solid samples.
Performing laser-induced fluorescence measurements on levitated samples.
Monitoring changes in solute concentration via solvent evaporation during levitation.

Main Results:

Acoustic levitation eliminates sample support interference, improving signal acquisition.
High concentrations of organic dyes can be measured without inner-filter effects.
Distance-dependent fluorescence modulations in semiconductor nanocrystals are studied with ease.

Conclusions:

Acoustic levitation offers a versatile, container-free platform for advanced fluorescence spectroscopy.
The technique overcomes key limitations of conventional methods, enabling new analytical possibilities.
It provides a simple yet powerful approach for studying nanomaterial fluorescence properties.