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Affinity Chromatography

Affinity Chromatography

Affinity chromatography is a powerful technique extensively utilized for separating and purifying specific biomolecules from complex mixtures. It capitalizes on the highly selective binding between an analyte and its counterpart, such as antibody-antigen interactions. The counterpart is immobilized on the stationary phase, forming an affinity column. The stationary phase typically consists of solid support, such as agarose or porous glass beads, immobilizing the affinity ligand. The mobile...

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Related Experiment Video

Updated: Jul 4, 2026

Protein Purification-free Method of Binding Affinity Determination by Microscale Thermophoresis

Protein Purification-free Method of Binding Affinity Determination by Microscale Thermophoresis

Published on: August 15, 2013

Control of an affinity purification procedure using a thermal biosensor.

L Flygare¹, P O Larsson, B Danielsson

¹Pure and Applied Biochemistry, Lund University, Box 124, S-221 00 Lund, Sweden.

Biotechnology and Bioengineering

|October 5, 1990

Summary

Lactate dehydrogenase (LDH) was recovered using affinity chromatography with AMP-Sepharose. An enzyme thermistor and PID controller accurately regulated adsorbent addition for efficient LDH recovery.

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Area of Science:

Biochemistry
Analytical Chemistry
Biotechnology

Background:

Lactate dehydrogenase (LDH) is an enzyme crucial in cellular metabolism.
Efficient recovery of LDH is important for diagnostic and research applications.
Affinity chromatography offers a selective method for protein purification.

Purpose of the Study:

To develop a method for the continuous and accurate recovery of lactate dehydrogenase (LDH).
To utilize an enzyme thermistor and automated control systems for optimizing adsorbent addition during affinity chromatography.

Main Methods:

Affinity binding of LDH to N(6)-(6-aminohexyl)-AMP-Sepharose gel.
Continuous monitoring of unbound LDH activity using an enzyme thermistor.
Automated control of adsorbent addition via a PID controller or desktop computer.

Main Results:

Both automated systems successfully regulated the addition of AMP-Sepharose gel.
The methods enabled rapid and accurate assessment of the correct adsorbent quantity.
Satisfactory recovery of lactate dehydrogenase was achieved.

Conclusions:

Enzyme thermistor-based feedback control provides an effective strategy for optimizing affinity chromatography.
Automated systems enhance the precision and efficiency of LDH recovery processes.
This approach facilitates accurate adsorbent addition for reliable enzyme purification.