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Related Experiment Video

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Following Cell-fate in E. coli After Infection by Phage Lambda

Following Cell-fate in E. coli After Infection by Phage Lambda

Published on: October 14, 2011

Two-stage fermentation with bacteriophage lambda as an expression vector in Escherachia coli.

T H Park¹, J H Seo, H C Lim

¹School of Chemical Engineering, Purdue University, West Lafayette, Indiana 47907, USA.

Biotechnology and Bioengineering

|February 20, 1991

Summary

Bacteriophage lambda shows promise as an expression vector for large-scale protein production. A temperature-sensitive mutant facilitated control, with a two-reactor system yielding promising results for cloned-gene expression.

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Area of Science:

Molecular Biology
Biotechnology
Bioprocess Engineering

Background:

Bacteriophage lambda is a virus that infects bacteria.
Expression vectors are used to produce proteins in host cells.
Controlling phage states (lysogenic/lytic) is key for protein production.

Purpose of the Study:

To evaluate bacteriophage lambda as an expression vector for large-scale protein production.
To utilize a temperature-sensitive mutant for controlled phage states.
To optimize protein yields using batch and continuous bioreactor systems.

Main Methods:

Utilized a temperature-sensitive mutant of bacteriophage lambda.
Employed batch and continuous bioreactor cultures.
Implemented a two-reactor system with temperature cycling for continuous culture.
Shifted temperature from 32°C to 38°C to induce lytic cycle.

Main Results:

Demonstrated bacteriophage lambda's potential as an expression vector.
Successfully controlled phage state using temperature shifts.
Achieved promising results in both batch and continuous systems.
Identified a Q gene deletion mutant as potentially more effective.

Conclusions:

Bacteriophage lambda is a viable candidate for large-scale protein expression.
Temperature-controlled induction offers a robust method for managing expression.
Further optimization with specific mutants may enhance protein yield and production efficiency.