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Updated: Jul 4, 2026

Atomic Absorbance Spectroscopy to Measure Intracellular Zinc Pools in Mammalian Cells
Published on: May 16, 2019
Graphite furnace atomic absorption spectrometry as a routine method for the quantification of beryllium in blood and
Chadi H Stephan1, Michel Fournier, Pauline Brousseau
1Department of Chemistry, Université de Montréal, Montréal, QC, Canada. chadi.stephan@umontreal.ca
Background:
A routine method for the quantification of beryllium in biological fluids is essential for the development of a chelation therapy for Chronic Beryllium Disease (CBD). We describe a procedure for the direct determination of beryllium in undigested micro quantities of human blood and serum using graphite furnace atomic absorption spectrometry. Blood and serum samples are prepared respectively by a simple 8-fold and 5-fold dilution with a Nash Reagent. Three experimental setups are compared: using no modifier, using magnesium nitrate and using palladium/citric acid as chemical modifiers.
Results:
In serum, both modifiers did not improve the method sensitivity, the optimal pyrolysis and atomization temperatures are 1000 degrees C and 2900 degrees C, respectively. In blood, 6 microg of magnesium nitrate was found to improve the method sensitivity. The optimal pyrolysis and atomization temperatures were 800 degrees C and 2800 degrees C respectively.
Conclusion:
In serum, the method detection limit was 2 ng l(-1), the characteristic mass was 0.22 (+/- 0.07) pg and the accuracy ranged from 95 to 100%. In blood, the detection limit was 7 ng l(-1), the characteristic mass was 0.20 (+/- 0.02) pg and the accuracy ranged from 99 to 101%.
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