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Synthesis of Keratin-based Nanofiber for Biomedical Engineering
Published on: February 7, 2016
Interactions between a poly(3-hydroxybutyrate-co-3-hydroxyvalerate-co-3-hydroxyhexanoate) terpolyester and human
Yan Ji1, Xiao-Tao Li, Guo-Qiang Chen
1Multidisciplinary Research Center, Shantou University, Shantou, Guangdong, China.
Biomaterials
|July 4, 2008
Summary
A novel polyhydroxyalkanoate, PHBVHHx, demonstrates superior biocompatibility for skin tissue engineering. Its unique properties enhance human keratinocyte growth, making it a promising biomaterial for skin regeneration applications.
Area of Science:
- Biomaterials Science
- Tissue Engineering
- Polymer Chemistry
Background:
- Polyhydroxyalkanoates (PHAs) are biodegradable polyesters with potential biomedical applications.
- Developing new PHA-based materials for skin tissue engineering requires understanding their interaction with skin cells.
- PHBVHHx, a terpolyester of 3-hydroxybutyrate (HB), 3-hydroxyvalerate (HV), and 3-hydroxyhexanoate (HHx), is a novel PHA candidate.
Purpose of the Study:
- To evaluate the suitability of PHBVHHx as a scaffold for skin tissue engineering.
- To investigate the biocompatibility and cell-growth promoting properties of PHBVHHx on human keratinocytes (HaCaT).
- To elucidate the mechanisms underlying PHBVHHx's superior cell support.
Main Methods:
- Comparative cell viability and proliferation assays of HaCaT cells on PHBVHHx films versus other biopolymers (PLA, PHB, PHBV, PHBHHx, P3HB4HB).
- Preparation and characterization of PHBVHHx nanoparticles (200-350nm).
- Assessment of cellular responses to PHBVHHx nanoparticles, including cytosolic calcium influx.
- Atomic Force Microscopy (AFM) analysis of PHBVHHx surface morphology and surface energy measurements.
Main Results:
- PHBVHHx films exhibited significantly higher HaCaT cell viability and growth compared to all tested biopolymers.
- PHBVHHx nanoparticles stimulated increased HaCaT cellular activity via enhanced cytosolic calcium influx.
- The degradation product 3-hydroxybutyrate (HB) also promoted HaCaT proliferation.
- PHBVHHx films displayed increased surface roughness and lower surface energy, correlating with enhanced cell adhesion and growth.
Conclusions:
- PHBVHHx demonstrates excellent biocompatibility and superior support for human keratinocyte growth.
- The observed cellular responses, including calcium influx and the role of HB, explain PHBVHHx's effectiveness.
- PHBVHHx is a highly promising material for the development of advanced skin tissue-engineered constructs.
