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Updated: Jul 3, 2026

Microplot Design and Plant and Soil Sample Preparation for 15Nitrogen Analysis
Published on: May 10, 2020
High enrichment of 15N by chromatographic chemical process
Xingcheng Ding1, Masao Nomura, Tatsuya Suzuki
1Institute of Nuclear Agricultural Science, Zhejiang University, Hangzhou 310029, China. dingxch@zju.edu.cn
Abstract:
Nitrogen isotope enrichment experiments were conducted to obtain highly enriched (15)N by ion-exchange process. (15)NH(4)Cl ((15)N=80%) as feeding materials were used to perform the chromatographic operation with two different flow rates and column diameters. Both separation coefficient (epsilon) and height equivalent to a theoretical plate (HETP) have same values in two run experiments. The value of HETP was more enlarged when high enrichment of (15)N was obtained in comparison with that of low enrichment. 99.756% (15)N and 13.63 g (15)N whose percentage was over 99.0% were successfully achieved by 25 m chromatographic migration with the flow rate and column diameter at 50 cm(3)/mL, 3.0 cm, respectively. High flow rate and large column diameter have advantages to the enrichment of (15)N by ion exchange process.
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