Study on the interaction of porphyrin with G-quadruplex DNAs
Related Experiment Video
Updated: Jul 3, 2026
Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes
Published on: April 4, 2025
Chunying Wei1, Gaoyi Han, Guoqing Jia
1Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Molecular Science, Shanxi University, Taiyuan 030006, China. weichuny@sxu.edu.cn
Abstract:
Interactions of 5,10,15,20-Tetrakis(N-propylpyridinium-4-yl)-21H,23H-porphyrin (TPrPyP4) with dimer hairpin (G4T4G4)2 and parallel four-stranded (TG4T)4 G-quadruplex DNAs in Na+-containing buffer were studied. The results show that two TPrPyP4 molecules bind to both G-quadruplexes by a noncooperative and nonequivalent binding mode, and there are one high affinity site and one low affinity site, the respective binding constants are 8.06 x 10(8) and 1.13 x 10(6) M(-1) for (G4T4G4)2-TPrPyP4, 8.04 x 10(7) and 9.08 x 10(5)M(-1) for (TG4T)4-TPrPyP4. TPrPyP4 presents two lifetimes of about 5.8 and 12.0 ns in the complexes of G-quadruplexes-TPrPyP4. The primary results suggest that two TPrPyP4 molecules bind to both G-quadruplexes by terminal stacking and outside binding mode.
