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DNA Microarrays: Sample Quality Control, Array Hybridization and Scanning
Published on: March 15, 2011
Gene expression profiling of experimental saccular aneurysms using deoxyribonucleic acid microarrays
1Neuroradiology Research Laboratory, Mayo Clinic College of Medicine, Rochester, MN 55905, USA.
AJNR. American Journal of Neuroradiology
|July 5, 2008
Summary
Gene expression in rabbit saccular aneurysms changes over time, with vessel wall remodeling genes up-regulated and inflammatory genes down-regulated. These findings offer insights into aneurysm pathophysiology.
Area of Science:
- Vascular Biology
- Molecular Medicine
- Genomics
Background:
- Saccular aneurysm pathophysiology is not fully understood at the molecular level.
- Investigating gene expression provides insights into disease mechanisms.
Purpose of the Study:
- To investigate gene expression patterns in elastase-induced saccular aneurysms in rabbits at different stages.
- To identify molecular changes associated with aneurysm development and progression.
Main Methods:
- Saccular aneurysms were created in rabbits using elastase.
- Deoxyribonucleic acid (DNA) microarrays were used to analyze gene expression at 2 and 12 weeks post-induction.
- Real-time polymerase chain reaction (RT-PCR) validated microarray findings.
Main Results:
- Significant differential gene expression was observed in aneurysm tissue compared to control carotid arteries.
- Genes involved in vessel wall remodeling were upregulated at both 2 and 12 weeks.
- Cell adhesion molecules and antioxidant enzymes were initially downregulated but normalized by 12 weeks; inflammatory and structural genes were generally underexpressed.
Conclusions:
- Multiple genes across diverse pathways are differentially expressed in this rabbit aneurysm model.
- These molecular changes contribute to the understanding of saccular aneurysm pathophysiology.
