[Arsenic trioxide induced apoptosis in retinoblastoma cells in vitro and its possible mechanism]
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Updated: Jul 3, 2026
Reconstruct Human Retinoblastoma In Vitro
Published on: October 11, 2022
Yun Li1, Luo-Sheng Tang, Hong-Wei Shen
1Department of Ophthalmology, Second Xiangya Hospital, Central South University, Changsha 410011, China. yunli0904@yahoo.com.cn
Objective:
To explore the effect of arsenic trioxide on the apoptosis of retinoblastoma cell line HXO-RB(44) and the possible mechanism.
Methods:
The effect of arsenic trioxide on the proliferation of retinoblastoma cell line HXO-RB(44) was observed by MTT colorimetric assay; the apoptosis of the HXO-RB(44) was examined by AO/EB staining and flow cytometry analysis (Annexin V+ PI staining); caspase-3 activity and bcl-2/bax expression in the HXO-RB(44) were detected by cpp32 colorimetric assay kit and Western blot.
Results:
Arsenic trioxide inhibited the proliferation of HXO-RB(44) cell in dose and duration-dependent manner in vitro; arsenic trioxide significantly increased the apoptosis in HXO-RB(44) cells. The activation of caspase-3 was increased, and the rate of bcl-2/bax was down-regulated in the HXO-RB(44) cells processed with arsenic trioxide.
Conclusion:
Arsenic trioxide can inhibit the proliferation of retinoblastoma cell HXO-RB(44) in vitro by apoptosis induction. The apoptosis induction is possibly related to the caspase-3 activation and bcl-2/bax down-regulation.
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