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Updated: Jul 3, 2026
Long-term Live-cell Imaging to Assess Cell Fate in Response to Paclitaxel
Published on: May 14, 2018
[Proteomic study of paclitaxel on human cervical carcinoma HCE1]
Lan-Qin Cao1, Qiu-Hua Lin, Xin Li
1Department of Gynecology and Obstetrics, Xiangya Hospital, Central South University, Changsha 410008, China. caolanqin@163.com
Objective:
To explore the mechanism of paclitaxel on the protein expression of human cervical carcinoma cell line HCE1.
Methods:
The total proteins extracted from paclitaxel-treated HCE1 cells were analyzed by 2-dimensional gel electrophoresis (2-DE), and compared with those from untreated HCE1 cells. The differential proteins were identified by mass spectrometry. Western blot was used to determine the differential expression levels of the 2 proteins.
Results:
At 24 hour after paclitaxel (0.05 mumol/L) treatment, 2-DE images of paclitaxel-treated and paclitaxel-untreated cells were analyzed. Forty-two differential proteins were found. Twenty-one differential proteins among 42 proteins were analyzed by mass spectrometry, among which 15 proteins were identified, including peptidyl-prolylisomerases A (PPIase A),alpha-enolase,keratin 8,heat shock protein 90, eukaryotic translation initiation factor 1A, and so on.
Conclusion:
Fifteen proteins in human cervical carcinoma cells paclitaxel-treated and paclitaxel-untreated are found by proteomic techniques. These proteins may be involved in the proliferation inhibition of human cervical carcinoma cells by paclitaxel.
