Multiple cytokine analysis in human tears: an optimized procedure for cytometric bead-based assay
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Updated: Jul 3, 2026
Bead Based Multiplex Assay for Analysis of Tear Cytokine Profiles
Published on: October 13, 2017
Martin W LaFrance1, Lucy E Kehinde, Roderick J Fullard
1School of Optometry, University of Alabama at Birmingham, Birmingham, Alabama, USA.
Purpose:
To compare instruments and reagents used for cytometric bead-based assay (CBA) of cytokines in human tears.
Methods:
Two CBA instruments and reagent kits from five vendors were compared in parallel. Vendor protocols were modified to accommodate tear volumes. Precision, recovery, dilution linearity, and stability were evaluated.
Results:
Luminextrade mark and Bio-Radtrade mark proved optimal for tear CBA. Good correlation for dilution linearity was observed (r > 0.80, p < 0.05) for 1-4-mu l sample volumes. Samples were stable for 30 min at 25 degrees C.
Conclusion:
Many tear cytokines can be reliably measured by CBA using an optimized protocol. Concentrations remain stable during periods consistent with collection.
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