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Published on: September 30, 2016
Adenosine kinase gene expression in human colorectal cancer
S Giglioni1, R Leoncini, E Aceto
1Dipartimento di Medicina Interna, Sc. Endocrino-Metaboliche e Biochimica, Università di Siena, Italy.
Abstract:
Real-time reverse transcription polymerase chain reaction (qRT-PCR) was used to evaluate gene expression of adenosine kinase, a key enzyme in adenosine metabolism, in human intestinal biopsy specimens of 10 colorectal cancer patients. Quantitative mRNA expression levels were normalized against the reference gene beta-actin. The results showed that adenosine kinase gene expression was significantly higher in cancer than in normal-appearing tissue, in line with our previous measurements of adenosine kinase enzyme activities in colorectal tumor samples.
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