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Activated Cross-linked Agarose for the Rapid Development of Affinity Chromatography Resins - Antibody Capture as a Case Study
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Affinity chromatography as a rapid and convenient method for purification of fungal laccases

J Rogalski1, M Wojtas-Wasilewska, R Apalovic

  • 1Department of Biochemistry, Maria Curie-Skłodowska University, 20-031 Lublin, Poland.

Biotechnology and Bioengineering
|April 5, 1991
PubMed

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Affinity Chromatography

Affinity chromatography is a powerful technique extensively utilized for separating and purifying specific biomolecules from complex mixtures. It capitalizes on the highly selective binding between an analyte and its counterpart, such as antibody-antigen interactions. The counterpart is immobilized on the stationary phase, forming an affinity column. The stationary phase typically consists of solid support, such as agarose or porous glass beads, immobilizing the affinity ligand. The mobile...