Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Biofilms01:29

Biofilms

Biofilms are complex communities of microorganisms encased in a self-produced extracellular polysaccharide matrix attached to surfaces. These microbial consortia can include single or multiple species, providing enhanced survival benefits by forming organized, multilayered structures.The formation of biofilms occurs through four key stages: attachment, colonization, development, and dispersal.During attachment, free-swimming planktonic cells adhere to a surface, often facilitated by...
Enzyme Kinetics01:19

Enzyme Kinetics

Enzymes speed up reactions by lowering the activation energy of the reactants. The speed at which the enzyme turns reactants into products is called the rate of reaction. Several factors impact the rate of reaction, including the number of available reactants. Enzyme kinetics is the study of how an enzyme changes the rate of a reaction.
Scientists typically study enzyme kinetics with a fixed amount of enzyme in the controlled environment of a test tube. When more reactant, or substrate, is...
Introduction to Enzyme Kinetics01:19

Introduction to Enzyme Kinetics

Enzyme kinetics studies the rates of biochemical reactions. Scientists monitor the reaction rates for a particular enzymatic reaction at various substrate concentrations. Additional trials with inhibitors or other molecules that affect the reaction rate may also be performed.
The experimenter can then plot the initial reaction rate or velocity (Vo) of a given trial against the substrate concentration ([S]) to obtain a graph of the reaction properties. For many enzymatic reactions involving a...
Fundamental Mathematical Principles in Pharmacokinetics: Rate and Order of Reaction01:15

Fundamental Mathematical Principles in Pharmacokinetics: Rate and Order of Reaction

In pharmacokinetics, the rates and order of reactions play a crucial role in understanding how the body processes drugs and help us comprehend drug absorption, distribution, metabolism, and elimination. A critical concept in pharmacokinetics is the rate constant, which quantifies the speed of a reaction. It provides valuable information about the kinetics of drug elimination. The rate constant allows us to determine the rate at which drugs are eliminated from the body.
Pharmacokinetic reactions...
Predicting Reaction Outcomes02:24

Predicting Reaction Outcomes

Kinetics describes the rate and path by which a reaction occurs. In contrast, thermodynamics deals with state functions and describes the properties, behavior, and components of a system. It is not concerned with the path taken by the process and cannot address the rate at which a reaction occurs. Although it does provide information about what can happen during a reaction process, it does not describe the detailed steps of what appears on an atomic or a molecular level. On the other hand,...
Reaction Rate02:53

Reaction Rate

The rate of reaction is the change in the amount of a reactant or product per unit time. Reaction rates are therefore determined by measuring the time dependence of some property that can be related to reactant or product amounts. Rates of reactions that consume or produce gaseous substances, for example, are conveniently determined by measuring changes in volume or pressure.
The mathematical representation of the change in the concentration of reactants and products, over time, is the rate...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Post-hoc analysis of a randomized controlled trial on the impact of pre-transplant use of probiotics on outcomes after liver transplantation.

Scientific reports·2020
Same author

Influence of Clonal Plasma Cell Contamination of Peripheral Blood Stem Cell Autografts on Progression and Survival in Multiple Myeloma Patients After Autologous Peripheral Blood Stem Cell Transplantation in Long-term Observation.

Transplantation proceedings·2018
Same author

Malnutrition Risk in Kidney Recipients Treated With Mycophenolate Mofetil Is Associated With IMPDH1 rs2278294 Polymorphism.

Transplantation proceedings·2018
Same author

Is Elective Status a Predictor of Poor Survival in Liver Retransplantation?

Transplantation proceedings·2017
Same author

Association of MYH9 rs3752462 and rs5756168 Polymorphisms With Transplanted Kidney Artery Stenosis.

Transplantation proceedings·2016
Same author

Profile of Gut Microbiota Associated With the Presence of Hepatocellular Cancer in Patients With Liver Cirrhosis.

Transplantation proceedings·2016
Same journal

Microbial Hydrolysates as Amino Acid Source in Cell Culture Media for Cellular Agriculture.

Biotechnology and bioengineering·2026
Same journal

LLM-Guided Parameter Optimization for Mechanistic CHO Cell Bioreactor Models.

Biotechnology and bioengineering·2026
Same journal

Three-Dimensional-Printed Polylactic Acid Scaffolds Coated With a Paeonol-Incorporated Gelatin/Bioactive Glass Composite Layer for Enhanced Osteogenic Performance.

Biotechnology and bioengineering·2026
Same journal

Recent Progress in Antimicrobial Peptides (AMPs) Towards Enhanced Selectivity and Reduced Cytotoxicity by Molecular Engineering.

Biotechnology and bioengineering·2026
Same journal

mZVI-Enhanced Mixed Nitrogen Removal in Klebsiella oxytoca via Coordinated Electron Transfer and Metabolic Reprogramming.

Biotechnology and bioengineering·2026
Same journal

Growth Model for Continuous Culture of a Hydrogen-Oxidizing Bacterium, Hydrogenophilus thermoluteolus Strain TH-1.

Biotechnology and bioengineering·2026
See all related articles

Related Experiment Video

Updated: Jul 3, 2026

Evaluation of the Efficacy of Organic Peroxyacids for Eradicating Dairy Biofilms Using an Approach Combining Static and Dynamic Methods
11:47

Evaluation of the Efficacy of Organic Peroxyacids for Eradicating Dairy Biofilms Using an Approach Combining Static and Dynamic Methods

Published on: December 9, 2022

Reaction kinetics in biofilms.

Z Lewandowski1, G Walser, W G Characklis

  • 1National Science Foundation Research Center for Interfacial Microbial Process Engineering, Montana State University, Bozeman, Montana 59717, USA.

Biotechnology and Bioengineering
|October 20, 1991
PubMed
Summary
This summary is machine-generated.

A new microtechnique measures biofilm parameters. This method quantifies dissolved oxygen diffusion, flux, and reaction rates within biofilms, advancing our understanding of microbial processes.

More Related Videos

Generation of Greater Bacterial Biofilm Biomass using PCR-Plate Deep Well Microplate Devices
10:57

Generation of Greater Bacterial Biofilm Biomass using PCR-Plate Deep Well Microplate Devices

Published on: April 22, 2022

Methodologies for Studying B. subtilis Biofilms as a Model for Characterizing Small Molecule Biofilm Inhibitors
10:17

Methodologies for Studying B. subtilis Biofilms as a Model for Characterizing Small Molecule Biofilm Inhibitors

Published on: October 9, 2016

Related Experiment Videos

Last Updated: Jul 3, 2026

Evaluation of the Efficacy of Organic Peroxyacids for Eradicating Dairy Biofilms Using an Approach Combining Static and Dynamic Methods
11:47

Evaluation of the Efficacy of Organic Peroxyacids for Eradicating Dairy Biofilms Using an Approach Combining Static and Dynamic Methods

Published on: December 9, 2022

Generation of Greater Bacterial Biofilm Biomass using PCR-Plate Deep Well Microplate Devices
10:57

Generation of Greater Bacterial Biofilm Biomass using PCR-Plate Deep Well Microplate Devices

Published on: April 22, 2022

Methodologies for Studying B. subtilis Biofilms as a Model for Characterizing Small Molecule Biofilm Inhibitors
10:17

Methodologies for Studying B. subtilis Biofilms as a Model for Characterizing Small Molecule Biofilm Inhibitors

Published on: October 9, 2016

Area of Science:

  • Microbiology
  • Biogeochemistry
  • Environmental Science

Background:

  • Biofilms are complex microbial communities crucial in various environments.
  • Understanding diffusion-controlled reactions within biofilms is key to predicting their behavior.
  • Previous methods lacked the resolution to accurately measure in situ biofilm parameters.

Purpose of the Study:

  • To develop and validate a novel in situ microtechnique for evaluating biofilm parameters.
  • To simultaneously measure dissolved oxygen concentration and optical density within biofilms.
  • To calculate key parameters of diffusion-controlled reactions in biofilms.

Main Methods:

  • Development of a novel in situ microtechnique.
  • Utilized a 15 mum diameter microprobe for measurements.
  • Simultaneously measured dissolved oxygen and optical density at varying biofilm depths.

Main Results:

  • Successfully evaluated parameters of diffusion-controlled reactions in biofilms.
  • Calculated the biofilm diffusion coefficient for dissolved oxygen (D(f)).
  • Determined the dissolved oxygen flux (J(02)) and half-velocity coefficient (K(s)).

Conclusions:

  • The novel microtechnique enables precise in situ evaluation of biofilm reaction parameters.
  • Quantified critical parameters like diffusion coefficient, flux, and reaction kinetics.
  • Provides a valuable tool for studying microbial processes in submerged biofilms.