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Related Experiment Videos

Hybridoma perfusion systems: a comparison study.

D de la Broise1, M Noiseux, B Massie

  • 1Croix Rouge Canadienne, Services Transfusionnels, Centre de Québec, Sainte-Foy, Canada.

Biotechnology and Bioengineering
|June 5, 1992
PubMed
Summary
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Hybridoma cell culture systems for IgM antibody production show comparable efficiency. However, cell loss in perfusion cultures significantly reduces antibody yield and per-cell productivity.

Area of Science:

  • Biotechnology
  • Cell Biology
  • Bioprocess Engineering

Background:

  • Hybridoma technology is crucial for monoclonal antibody production.
  • Perfusion culture systems offer advantages for long-term cell culture and antibody yield.
  • Optimizing cell retention and productivity in bioreactors is essential.

Purpose of the Study:

  • To compare the efficiency of IgM production in hybridoma cells across different perfusion culture systems.
  • To evaluate the impact of cell retention and loss on antibody yield and productivity.
  • To validate a "grow or die" cell cycle model in hybridoma perfusion cultures.

Main Methods:

  • Long-term perfusion cultures of hybridoma cells.
  • Comparison of three culture systems: suspension, alginate beads, and hollow-fiber cartridge bioreactors.

Related Experiment Videos

  • Monitoring of steady-state cell concentration and IgM production rates.

    • Main Results:

    • Similar steady-state cell concentrations and antibody production per liter were observed in suspension, alginate beads, and hollow-fiber systems.
    • Significant reduction in antibody production occurred when viable cells were lost in the filtrate.
    • Cell loss led to decreased viable cell yield and lower per-cell IgM productivity.

    Conclusions:

    • Cell behavior and antibody yield are comparable across different hybridoma perfusion systems.
    • Minimizing viable cell loss in perfusion filtrate is critical for maximizing antibody production.
    • Cell retention strategies in bioreactors, potentially by modulating the "grow or die" cell cycle, can enhance IgM productivity.