Recovery of canola meal proteins by precipitation

Recovery of rapeseed proteins from defatted canola meal by precipitation was investigated. The ability of different precipitating agents, such as sodium hexametaphosphate (HMP), carboxymethylcellulose (CMC), ammonium sulphate, and isoelectric precipitation using HCl, were evaluated based on the yield and mean size of protein aggregates. Almost 94% of dissolved protein was precipitated in the presence of 2.7 M ammonium sulphate, while the largest mean protein particle size (32 microm) was obtained in the presence of HMP at pH 3.3.