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Updated: Jul 3, 2026

A Polyaniline-based Sensor of Nucleic Acids

A Polyaniline-based Sensor of Nucleic Acids

Published on: November 1, 2016

Urea sensor with single poly(propylene) membrane.

Y J Wang¹, C H Chen, G H Hsiue

¹Institute of Biomedical Engineering, Institute of Biochemistry, National Yang-Ming Medical College, Taipei, Taiwan, Republic of China.

Biotechnology and Bioengineering

|July 1, 1992

Summary

New urea sensors utilize a novel immobilized urease on a plasma-aminated hydrophobic poly(propylene) membrane. This design offers improved response times and stable sensitivity for urea detection.

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Published on: November 22, 2016

Area of Science:

Biomaterials Science
Analytical Chemistry
Sensor Technology

Background:

Enzyme immobilization is crucial for biosensor development.
Poly(propylene) membranes offer unique surface properties for biomolecule attachment.
Developing efficient and stable urea sensors is important for various applications.

Purpose of the Study:

To immobilize urease onto a plasma-aminated hydrophobic poly(propylene) membrane.
To construct and characterize a novel urea sensor using this modified membrane.
To evaluate the sensor's performance, including response time, sensitivity, and stability.

Main Methods:

Plasma amination of hydrophobic poly(propylene) membranes.
Immobilization of urease enzyme onto the modified membrane surface.
Fabrication of a single-membrane enzyme electrode for urea sensing.
Electrochemical characterization of the urea sensor's response to varying urea concentrations, pH, and buffer strength.

Main Results:

Urease successfully immobilized on plasma-aminated hydrophobic poly(propylene) membranes.
The developed urea sensors exhibited response sensitivities between 19 mV/decade and 30 mV/decade.
The single-membrane enzyme electrode demonstrated shorter response times compared to conventional electrodes.
Sensor sensitivity was pH-dependent, peaking at pH 7.6 (40 mV/decade), and unaffected by buffer strength.
The urea electrode showed operational stability for up to 12 days.

Conclusions:

Plasma-aminated hydrophobic poly(propylene) membranes are suitable supports for urease immobilization.
The novel single-membrane urea sensor design offers advantages in response time and stability.
The sensor's performance is optimized at a neutral pH (7.6).
This approach provides a promising platform for developing robust and efficient urea biosensors.