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Updated: Jul 3, 2026

A Sensitive Visual Method for the Detection of Hydrogen Sulfide Producing Bacteria
Published on: June 27, 2022
Effect of hydrogen sulfide on growth of sulfate reducing bacteria
M A Reis1, J S Almeida, P C Lemos
1Lab. de Engenharia Bioquímica, Faculdade de Ciências e Tecnologia/UNL, 2825 Monte da Caparica, Portugal.
Abstract:
A culture of sulfate reducing bacteria (SRB) growing on lactate and sulfate was incubated at different pH values in the range of 5.8-7.0. The effect of pH on growth rate was determined in this pH range; the highest growth rate was observed at pH 6.7. Hydrogen sulfide produced from sulfate reduction was found to have a direct and reversible toxicity effect on the SRB. A hydrogen sulfide Concentration of 547 mg/L (16.1 mM) completely inhibited the culture growth. Comparison between acetic acid and hydrogen sulfide inhibition is presented and the concomitant inhibition kinetics are mathematically described.
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