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Updated: Jul 3, 2026
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Defining Substrate Specificities for Lipase and Phospholipase Candidates
Published on: November 23, 2016
Cellulase activity in commercial lipase preparations
J T Derksen1, G Boswinkel, F P Cuperus
1Agrotechnological Research Institute, Wageningen, NL-6700 AA, The Netherlands.
Biotechnology and Bioengineering
|October 5, 1992
Summary
Cellulose membranes used in bioreactors for enzyme immobilization are degraded by cellulase found in lipase preparations. This research highlights cellulose membrane unsuitability for lipase immobilization, prompting the development of alternatives.
Area of Science:
- Biotechnology
- Materials Science
- Enzyme Engineering
Background:
- Polymeric membranes are widely utilized for enzyme immobilization in bioreactors.
- Modified cellulose membranes are common supports for lipase-catalyzed oil hydrolysis.
Purpose of the Study:
- To evaluate the suitability of cellulose membranes as supports for lipase immobilization in bioreactors.
- To identify potential issues arising from the interaction between lipase preparations and cellulose membranes.
Main Methods:
- Investigated the stability of modified cellulose membranes when used with commercial lipase preparations.
- Assessed membrane integrity and reactor performance under operational conditions.
Main Results:
- Commercial lipase preparations containing cellulase readily attacked and degraded cellulose membranes.
- Degradation resulted in significant membrane damage, leading to bioreactor instability and leakage.
Conclusions:
- Cellulose membranes are unsuitable for immobilizing lipases due to cellulase-induced degradation.
- Alternative membrane materials are required for stable and efficient lipase-based bioreactors.
