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Fluorescence Recovery after Photobleaching of Yellow Fluorescent Protein Tagged p62 in Aggresome-like Induced Structures
12:58

Fluorescence Recovery after Photobleaching of Yellow Fluorescent Protein Tagged p62 in Aggresome-like Induced Structures

Published on: March 26, 2019

A reducing and oxidizing system minimizes photobleaching and blinking of fluorescent dyes

Jan Vogelsang1, Robert Kasper, Christian Steinhauer

  • 1Angewandte Physik-Biophysik, and Center for NanoScience, Ludwig-Maximilians-Universität, Amalienstrasse 54, 80799 München, Germany.

Angewandte Chemie (International Ed. in English)
|July 8, 2008
PubMed
Abstract

No abstract available in PubMed .

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Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

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