Pulsatile flow filtration of yeast cell debris: influence of preincubation on performance

The recovery of proteins from yeast cell debris suspension was investigated using a vortex mixing technique based on the combination of oscillatory flow and a baffled flat-sheet microfiltration system. For this system, increased protein transmission was obtained through the use of low transmembrane pressures and the preincubation of the yeast cell debris feed suspension at 30 degrees C. Furthermore, a plateau in the flux-time curve was observed. In the absence of baffles and pulsations, preincubation had a little effect.