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Related Concept Videos

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Bioluminescent Bacterial Imaging In Vivo
05:06

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Published on: November 4, 2012

A novel BOD sensor based on bacterial luminescence.

C K Hyun1, E Tamiya, T Takeuchi

  • 1Research Center for Advanced Science and Technology, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153, Japan.

Biotechnology and Bioengineering
|May 1, 1993
PubMed
Summary

A novel biosensor using luminous bacteria, Photobacterium phosphoreum, rapidly measures biochemical oxygen demand (BOD) in 15 minutes with high accuracy. This innovative bacterial reagent offers a quick and reliable method for environmental water quality assessment.

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Area of Science:

  • Environmental Science
  • Biotechnology
  • Analytical Chemistry

Background:

  • Conventional biochemical oxygen demand (BOD) measurements are time-consuming, typically requiring 5 days.
  • There is a need for rapid and accurate methods to assess water quality and organic pollution.
  • Bioluminescent bacteria offer a potential platform for developing novel biosensors due to their sensitivity to environmental changes.

Purpose of the Study:

  • To develop a novel reagent-type BOD sensor utilizing the luminous bacterium Photobacterium phosphoreum.
  • To evaluate the performance and applicability of the developed bacterial reagent for BOD measurement.
  • To establish practical preservation methods for the bacterial reagent.

Main Methods:

  • Development of a BOD sensor based on the luminescence of Photobacterium phosphoreum, detecting changes in light intensity due to organic compound assimilation.
  • Optimization of measurement conditions, including temperature (18-25°C) and pH (7-8).
  • Investigation of long-term preservation methods: vacuum drying and freezing.
  • Comparative analysis of BOD values obtained from the sensor against conventional 5-day BOD tests for various organic substrates and wastewater samples.

Main Results:

  • The developed BOD sensor provides results within 15 minutes with an error of +/-7%.
  • Optimal measurement conditions were found to be room temperature (18-25°C) and neutral to slightly alkaline pH (7-8).
  • Both vacuum drying and freezing methods proved effective for long-term preservation of the bacterial reagent.
  • BOD values estimated by the sensor showed good agreement with conventional 5-day BOD measurements for wastewater samples.

Conclusions:

  • A rapid and accurate reagent-type BOD sensor using Photobacterium phosphoreum has been successfully developed.
  • The sensor operates effectively at room temperature, simplifying measurement procedures.
  • Practical preservation techniques ensure the reagent's viability for long-term use.
  • This bacterial biosensor offers a promising alternative to conventional methods for environmental monitoring of organic pollution.