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Related Experiment Video

Updated: Jul 3, 2026

Evaluation of Lung Metastasis in Mouse Mammary Tumor Models by Quantitative Real-time PCR
08:56

Evaluation of Lung Metastasis in Mouse Mammary Tumor Models by Quantitative Real-time PCR

Published on: January 29, 2016

Reference gene selection for real-time RT-PCR in regenerating mouse livers.

Kohei Tatsumi1, Kazuo Ohashi, Sanae Taminishi

  • 1Department of Pediatrics, Nara Medical University, 840 Shijo-cho, Kashihara, Nara 634-8522, Japan.

Biochemical and Biophysical Research Communications
|July 8, 2008
PubMed
Summary

Identifying stable housekeeping genes (HKGs) is crucial for accurate liver regeneration research. PPIA and TBP were found to be the most reliable internal controls for gene expression analysis during liver repair.

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Area of Science:

  • Hepatology
  • Molecular Biology
  • Genomics

Background:

  • The liver possesses a remarkable capacity for regeneration following injury.
  • Liver regeneration involves intricate changes in gene expression profiles.
  • Accurate quantification of gene expression in regenerating liver requires stable housekeeping genes (HKGs).

Purpose of the Study:

  • To evaluate the stability of commonly used HKGs during liver regeneration.
  • To identify ideal HKGs for normalizing gene expression data in regenerating liver models.

Main Methods:

  • Studied the gene expression of 8 HKGs (GAPDH, ACTB, HPRT1, GUSB, PPIA, TBP, TFRC, RPL4) in quiescent and regenerating mouse livers.
  • Induced liver regeneration via partial hepatectomy.
  • Analyzed HKG stability using geNorm and NormFinder algorithms.

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Cell Type-specific Gene Expression Profiling in the Mouse Liver
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Published on: September 17, 2019

Related Experiment Videos

Last Updated: Jul 3, 2026

Evaluation of Lung Metastasis in Mouse Mammary Tumor Models by Quantitative Real-time PCR
08:56

Evaluation of Lung Metastasis in Mouse Mammary Tumor Models by Quantitative Real-time PCR

Published on: January 29, 2016

Using Quantitative Real-time PCR to Determine Donor Cell Engraftment in a Competitive Murine Bone Marrow Transplantation Model
10:11

Using Quantitative Real-time PCR to Determine Donor Cell Engraftment in a Competitive Murine Bone Marrow Transplantation Model

Published on: March 7, 2013

Cell Type-specific Gene Expression Profiling in the Mouse Liver
10:06

Cell Type-specific Gene Expression Profiling in the Mouse Liver

Published on: September 17, 2019

Main Results:

  • PPIA and TBP exhibited the most stable expression across different stages of liver regeneration.
  • Other commonly used HKGs showed significant variations in expression levels.

Conclusions:

  • PPIA and TBP are validated as reliable housekeeping genes for accurate normalization in liver regeneration studies.
  • These findings will enhance the reliability of gene expression analysis in hepatology research.