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Updated: Jul 3, 2026
Broth Microdilution In Vitro Screening: An Easy and Fast Method to Detect New Antifungal Compounds
Published on: February 14, 2018
Subculture on potato dextrose agar as a complement to the broth microdilution assay for Malassezia pachydermatis
Marilena R Prado1, Erika H S Brito, Raimunda S N Brilhante
1Faculty of Veterinary Medicine, Program of Post-Graduation in Veterinary Science, State University of Ceará, Fortaleza, Ceará, Brazil. lena@inteq.com.br
Abstract:
The main aim of this study was to verify the efficacy of subculture on potato dextrose agar (PDA) as a complement to the in vitro susceptibility test for Malassezia pachydermatis strains by a broth microdilution method, as well as to determine the MIC and MFC of azole derivatives, amphotericin B and caspofungin. The microdilution assay was performed in 96-well plates using a modified RPMI 1640 medium. The M. pachydermatis strains were resistant to caspofungin. All strains (n=50) had shown MIC values of <0.03, <0.03, 2.0, 4.0 and 4.0 microg/ml for itraconazole, ketoconazole, voriconazole, fluconazole and amphotericin B, respectively. Thus, the subculture on PDA improved the analysis of the in vitro antifungal susceptibility of M. pachydermatis.
