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Updated: Jul 3, 2026
Tissue Characterization after a New Disaggregation Method for Skin Micro-Grafts Generation
Published on: March 4, 2016
Microbiological assessment of cadaver skin grafts received in a Skin Bank
Meenakshi Mathur1, Anuradha De, Madhuri Gore
1Department of Microbiology, Lokmanya Tilak Municipal Medical College and General Hospital, Sion, Mumbai, India.
Purpose:
Study on the microbiological assessment of cadaver skin grafts was undertaken in the Skin Bank at the Department of Surgery in L.T.M. Medical College and Hospital, Mumbai, India.
Procedure:
Microbiology of the skin grafts was studied immediately on receipt and after antibiotic treatment. The antimicrobial and antifungal agents used were crystalline penicillin, gentamicin, amikacin, ceftazidime and amphotericin B.
Findings:
Escherichia coli, Staphylococcus aureus and Candida species were the predominant microorganisms isolated on pre-processing cultures from cadaver skin grafts. Seventy percent of pre-processing cultures showed growth. After first cycle antibiotic treatment, 52.4% of grafts showing growth became sterile. Growth of Klebsiella pneumoniae persisted in two cases, even after 2nd cycle of antibiotic treatment. After the use of antifungal, 57.1% of those showing fungal growth became sterile.
Conclusion:
Various antibiotics and antifungals can be used in order to minimize the discard rate of skin grafts.
