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Cryo-electron Microscopy01:28

Cryo-electron Microscopy

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Imaging cryosurgery with EIT: tracking the ice front and post-thaw tissue viability.

Jon F Edd1, Antoni Ivorra, Liana Horowitz

  • 1Department of Mechanical Engineering, University of California, Berkeley, CA 94720, USA.

Physiological Measurement
|July 8, 2008
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Electrical impedance tomography (EIT) can monitor cell death during cryosurgery. This study demonstrates EIT

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Area of Science:

  • Biomedical Engineering
  • Medical Imaging
  • Cryobiology

Background:

  • Cryosurgery uses freezing to destroy unwanted tissues like tumors.
  • Current ice front imaging is insufficient as some frozen tissue survives.
  • Monitoring cryosurgery's treatment effect requires assessing cell death.

Purpose of the Study:

  • To investigate the use of Electrical Impedance Tomography (EIT) for monitoring cryosurgery.
  • To assess EIT's capability in imaging ice fronts and detecting cell death in vivo.
  • To validate EIT imaging with histological data in a preclinical model.

Main Methods:

  • Developed and tested a 3D EIT system for cryosurgery monitoring.
  • Performed ice front imaging tests using a gel phantom.
  • Conducted in vivo experiments in a rat liver model during freeze-thaw cycles.
  • Validated EIT results with post-thaw viability imaging in potato and histological analysis.

Main Results:

  • Successfully imaged ice fronts in a gel phantom.
  • Demonstrated 3D differential conductivity imaging throughout the freeze-thaw cycle in vivo.
  • Showcased EIT's potential for assessing tissue viability post-cryosurgery.

Conclusions:

  • EIT can image ice fronts and monitor cryoablation effects in real-time.
  • EIT shows promise for enabling iterative cryosurgery by assessing treatment efficacy.
  • In vivo EIT imaging of cryosurgery is feasible and warrants further investigation.