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Affinity partitioning of vancomycin.

P P Godbole1, R S Tsai, W M Clark

  • 1Chemical Engineering Department, Worcester Polytechnic Institute, Worcester, Massachusetts 01609, USA.

Biotechnology and Bioengineering
|August 20, 1991
PubMed
Summary
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This study explores affinity partitioning for vancomycin separation. The Flanagan and Barondes model offers qualitative predictions, but optimizing polymer-ligand properties is key for efficient antibiotic recovery from fermentation broths.

Area of Science:

  • Biochemistry
  • Separation Science
  • Chemical Engineering

Background:

  • Affinity partitioning is a technique used for designing separation systems.
  • Vancomycin, a glycopeptide antibiotic, requires effective separation methods.
  • Existing models provide a basis for understanding partitioning behavior.

Purpose of the Study:

  • To evaluate the Flanagan and Barondes model for affinity partitioning of vancomycin.
  • To investigate the influence of ligand properties on separation efficiency.
  • To develop an improved process for vancomycin recovery.

Main Methods:

  • Experimental affinity partitioning using vancomycin in a water-methoxypolyethylene glycol-dextran system.
  • Utilizing methoxypolyethylene glycol bound D-alanyl-D-alanine or D-alanyl-D-alanine as affinity ligands.

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  • Independent measurement of model parameters and experimental variation.

    • Main Results:

    • The Flanagan and Barondes model provided only qualitative predictions for 1:1 binding interactions.
    • Discrepancies were attributed to differences in exposed surfaces between free and bound states.
    • Enhancement of the system requires polymer-ligands with stronger partitioning to the top phase, achievable by increasing molecular weight or hydrophobicity.

    Conclusions:

    • Optimizing polymer-ligand characteristics is crucial for enhancing affinity partitioning systems.
    • Increased polymer-ligand molecular weight or hydrophobicity improves separation efficiency.
    • A process for vancomycin recovery from fermentation broth using D-alanyl-D-alanine sepharose was successfully described.