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Efficient Chromatin Immunoprecipitation using Limiting Amounts of Biomass
Published on: May 1, 2013
[Analysis of appropriate amount of template DNA for sinofiler kit by real time quantitative PCR technique]
Cheng-Tao Li1, Hong Guo, Yuan Lin
1Shanghai Key Laboratory of Forensic Medicine, Institute of Forensic Science, Ministry of Justice, P.R. China. lichengtaohla@163.com
Objective:
To explore the appropriate amount of template DNA for Sinofiler Kit.
Methods:
The DNA samples with ideally genotyped results by Sinofiler Kit were detected by real-time quantitative PCR assay.
Results:
It was shown that 1.29-1.51 ng of template DNA in 12.5 microL reaction volume was optimal for STR genotyping with Sinofiler Kit.
Conclusion:
Real time quantitative PCR is an accurate and necessary technique for detection of appropriate amount of template DNA for different kits.
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