Tumor-specific humoral antibodies against plasma cell tumors in immunized BALB/c mice

In BALB/c mice immunized with plasma cell tumors incubated in sodium iodoacetate, antibodies against plasma cell tumors were detected by the indirect fluorescent antibody test. Fluorescence indices were determined in individual sera of animals bled at different intervals. After completion of immunizing injections, the time of appearance, level, and duration of antibodies depended on the number of injections. Cross-reactions and cross-absorption of antisera with several transplant lines of plasma cell tumor detected antibodies against individual tumor antigens and antibodies against antigen common to several plasma cell tumors. A relationship between antibody level, as detected by the fluorescence index, and resistance of animals to tumor graft could not be established.