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Rapid screening by real-time 16S rDNA PCR for bacterial contamination of blood products

Hendrik W Reesink1, Tamimount Mohammadi, Rubyn N I Pietersz

  • 1Sanquin Blood Bank North-West Region and Sanquin Diagnostic Services, Amsterdam, The Netherlands. h.reesink@sanquin.nl

Clinical Chemistry and Laboratory Medicine
|July 9, 2008
PubMed

Insights

Bacterial contamination in platelet concentrates can cause adverse transfusion events. This review focuses on real-time polymerase chain reaction (PCR) for detecting bacterial 16S rDNA in these products.

Area of Science:

  • Microbiology
  • Transfusion Medicine
  • Molecular Diagnostics

Background:

  • Blood component transfusion, while generally safe, carries risks of adverse events, including infectious complications.
  • Blood-borne infections from transfused products remain a significant concern for patient safety.
  • Platelet concentrates are particularly susceptible to bacterial contamination.

Purpose of the Study:

  • To review methods for detecting bacterial contamination in platelet concentrates.
  • To specifically discuss real-time polymerase chain reaction (PCR) for bacterial detection.
  • To highlight the application of real-time 16S rDNA detection in this context.

Main Methods:

  • Review of existing literature on bacterial detection methods in platelet concentrates.
  • Focus on molecular techniques, specifically real-time PCR.
  • Detailed examination of real-time 16S rDNA detection assays.

Main Results:

  • Various methods exist for bacterial detection in platelet concentrates.
  • Real-time PCR offers a sensitive approach for identifying bacterial contaminants.
  • 16S rDNA real-time PCR is a promising technique for rapid and accurate detection.

Conclusions:

  • Bacterial contamination detection in platelet concentrates is crucial for transfusion safety.
  • Real-time PCR, particularly 16S rDNA detection, represents an advanced method for this purpose.
  • Further investigation and implementation of such methods can enhance the safety of blood products.

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