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Intravital Imaging of Intraepithelial Lymphocytes in Murine Small Intestine
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Lambda light chain revision in the human intestinal IgA response.

Wen Su1, John N Gordon, Francesca Barone

  • 1Department of Immunobiology, Kings College London School of Medicine, Guy's King's College, St Thomas' Hospitals, London, United Kingdom.

Journal of Immunology (Baltimore, Md. : 1950)
|July 9, 2008
PubMed
Summary
This summary is machine-generated.

Antibody L chain revision occurs in human intestinal IgA plasma cells, altering immune responses. This process, particularly involving lambda L chains, diversifies antibody specificity in this major antibody-producing population.

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Area of Science:

  • Immunology
  • Molecular Biology
  • Cell Biology

Background:

  • Secondary rearrangement of antibody (Ab) light (L) chains in mature B cells can alter immune response targets.
  • Intestinal IgA plasma cells (PCs) represent the largest Ab-producing population in humans.

Purpose of the Study:

  • To investigate whether L chain revision occurs in human intestinal IgA plasma cells.
  • To determine the extent and mechanism of L chain revision in this specific B cell population.

Main Methods:

  • Analysis of lambda L chain repertoire biases (productive and non-productive).
  • Identification of circular DNA products resulting from rearrangement.
  • Detection of RAG genes and associated proteins.

Main Results:

  • Light chain revision is a normal and widespread process in intestinal IgA plasma cells.
  • Specific biases in lambda L chain repertoire and detection of revision products confirm L chain revision.
  • No significant evidence of immunoglobulin heavy (IgH) chain revision was observed.
  • Limited evidence of kappa-chain revision suggests locus inactivation.

Conclusions:

  • The lambda L chain locus is accessible and plays a key role in modifying and diversifying Ab specificity within intestinal IgA PCs.
  • L chain revision is a critical mechanism for immune adaptation and specificity in the gut.