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Related Experiment Videos

Does regional norepinephrine spillover represent local sympathetic activity?

P C Chang1, E Kriek, J A van der Krogt

  • 1Department of Nephrology, University of Leiden, The Netherlands.

Hypertension (Dallas, Tex. : 1979)
|July 1, 1991
PubMed
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Regional spillover of norepinephrine (NE) is influenced by local clearance and blood flow. A new parameter, plasma appearance rate of NE, may better reflect NE entry into the blood, unaffected by these changes.

Area of Science:

  • Pharmacology
  • Physiology
  • Cardiovascular Research

Background:

  • Regional spillover of norepinephrine (NE) is a key indicator of sympathetic activity.
  • Previous studies have not fully explored the impact of local NE clearance on spillover measurements.

Purpose of the Study:

  • To investigate the effects of local changes in norepinephrine clearance and forearm blood flow on NE spillover.
  • To evaluate a new parameter, plasma appearance rate of NE, as a potentially more accurate measure of NE entry into circulation.

Main Methods:

  • Utilized isotope dilution techniques with intra-arterial infusions of NE, tritiated NE, desipramine (neuronal uptake inhibitor), and tyramine in 10 healthy subjects.
  • Measured NE kinetics and clearance in the forearm under conditions of blocked neuronal uptake and altered blood flow.

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Main Results:

  • Neuronal uptake blockade by desipramine significantly decreased forearm NE clearance (59%) and intrinsic clearance capacity (65%), while increasing NE spillover.
  • Increased forearm blood flow (nitroprusside infusion) enhanced NE spillover and NE clearance, leaving intrinsic clearance capacity unchanged.
  • The proposed plasma appearance rate of NE showed nonsignificant changes under both desipramine and nitroprusside infusions.

Conclusions:

  • Regional spillover of NE is significantly influenced by local clearance and blood flow dynamics.
  • The plasma appearance rate of NE may offer a more reliable estimation of NE's regional entry into the blood pool compared to spillover.
  • These findings necessitate a re-evaluation of NE spillover as a sole indicator of sympathetic activity.