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Selective targeting of pigmented retinal pigment epithelial (RPE) cells by a single pulsed laser irradiation: an in
1The Center for Engineering in Medicine, Massachusetts General Hospital, Harvard Medical School and ShrinersBurns Hospital, 51 Blossom Street, Boston, MA 02114, USA.
Abstract:
This work describes the selective targeting of pigmented retinal pigment epithelial (RPE) cells by a single pulsed laser irradiation. We observed: (1) single pulsed laser irradiation caused cellular damages on pigmented, and not on non-pigmented RPE cells at laser radiant exposure up to 2550 mJ/cm(2); (2) in the mixture of pigmented and non-pigmented RPE cells, single pulsed laser-induced damage was confined to pigmented RPE cells. This study demonstrates that the pigmented RPE cells can be selectively damaged, using a single pulsed laser irradiation, without thermal coagulation to adjacent non-pigmented RPE cells.
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