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Related Concept Videos

Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
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Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
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Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
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Super-resolution Fluorescence Microscopy

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Total Internal Reflection Fluorescence Microscopy

Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.

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Related Experiment Video

Updated: Jul 3, 2026

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy (oSLO) and Optical Coherence Tomography (OCT)
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Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy (oSLO) and Optical Coherence Tomography (OCT)

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Compact Er:Yb:glass-laser-based supercontinuum source for high-resolution optical coherence tomography.

M C Stumpf1, S C Zeller, A Schlatter

  • 1Department of Physics, Institute of Quantum Electronics, ETH Zurich, 8093 Zurich, Switzerland. stumpf@phys.ethz.ch

Optics Express
|July 9, 2008
PubMed
Summary

We developed a super-continuum light source using a diode-pumped laser, generating ultrashort pulses. This technology enables ultrahigh-resolution optical coherence tomography (OCT) with 3.5 micrometer axial resolution.

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Last Updated: Jul 3, 2026

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy (oSLO) and Optical Coherence Tomography (OCT)
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20 mJ, 1 ps Yb:YAG Thin-disk Regenerative Amplifier
10:17

20 mJ, 1 ps Yb:YAG Thin-disk Regenerative Amplifier

Published on: July 12, 2017

Area of Science:

  • Photonics and Laser Technology
  • Optical Metrology

Background:

  • Super-continuum generation is crucial for broadband light sources.
  • Diode-pumped, passively mode-locked lasers offer compact and efficient pulse generation.
  • Achieving octave-spanning spectra is key for advanced optical applications.

Purpose of the Study:

  • To develop a low-coherence light source for ultrahigh-resolution optical coherence tomography (OCT).
  • To demonstrate super-continuum generation from a diode-pumped, passively mode-locked Er:Yb:glass laser.
  • To characterize the spectral broadening and coherence properties of the generated light.

Main Methods:

  • Direct super-continuum generation using a diode-pumped, passively mode-locked Er:Yb:glass laser.
  • Launching 198 fs transform-limited pulses into a dispersion-optimized highly nonlinear fiber.
  • Spectral broadening to achieve an optical bandwidth spanning 1150 nm to 2400 nm (more than one octave).

Main Results:

  • Generation of 198 fs transform-limited pulses with 100 mW average power at 75 MHz repetition rate.
  • Achieved an octave-spanning optical bandwidth from 1150 nm to 2400 nm.
  • Coherence measurements demonstrated potential for 3.5 micrometer axial resolution in air for OCT.

Conclusions:

  • The developed super-continuum light source provides a robust platform for ultrahigh-resolution OCT.
  • The broad spectral bandwidth and coherence properties are suitable for advanced imaging applications.
  • This work highlights the potential of integrated laser and nonlinear fiber systems for metrology.