Related Experiment Video
Updated: Jul 3, 2026
Purification of Pathogen Vacuoles from Legionella-infected Phagocytes
Published on: June 19, 2012
Purification and characterization of laccase secreted by L. lividus
R Sahay1, R S S Yadav, K D S Yadav
1Department of Chemistry, D.D.U. Gorakhpur University, Gorakhpur 273 009, India.
Abstract:
The culture conditions for maximum secretion of laccase by Loweporus lividus MTCC-1178 have been optimized. The laccase from the culture filtrate of L. lividus MTCC-1178 has been purified to homogeneity. The molecular weight of the purified laccase is 64.8 kDa. The enzymatic characteristics like K(m), pH, and temperature optimum using 2,6-dimethoxyphenol have been determined and found to be 480 microM, 5.0, and 60 degrees C, respectively. The K(m) values for other substrates like catechol, m-cresol, pyrogallol, and syringaldazine have also been determined and found to be 230, 210, 320, and 350 microM, respectively.
More Related Videos
05:31Purification and Visualization of Lipopolysaccharide from Gram-negative Bacteria by Hot Aqueous-phenol Extraction
Published on: May 28, 2012
08:29Au-Interaction of Slp1 Polymers and Monolayer from Lysinibacillus sphaericus JG-B53 - QCM-D, ICP-MS and AFM as Tools for Biomolecule-metal Studies
Published on: January 19, 2016
