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Updated: Jul 3, 2026

Combining Analysis of DNA in a Crude Virion Extraction with the Analysis of RNA from Infected Leaves to Discover New Virus Genomes
Published on: July 27, 2018
Identification of viral and non-viral reverse transcribing elements in pineapple (Ananas comosus), including members
C F Gambley1, A D W Geering, V Steele
1Department of Primary Industries and Fisheries, Horticulture and Forestry Science, 80 Meiers Road, Indooroopilly, Queensland 4068, Australia. cherie.gambley@dpi.qld.gov.au
Abstract:
A previously published partial sequence of pineapple bacilliform virus was shown to be from a retrotransposon (family Metaviridae) and not from a badnavirus as previously thought. Two newly discovered sequence groups isolated from pineapple were associated with bacilliform virions and were transmitted by mealybugs. Phylogenetic analyses indicated that they were members of new badnavirus species. A third caulimovirid sequence was also amplified from pineapple, but available evidence suggests that this DNA is not encapsidated, but more likely derived from an endogenous virus.
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