Related Experiment Video
Updated: Jul 3, 2026

Artificial RNA Polymerase II Elongation Complexes for Dissecting Co-transcriptional RNA Processing Events
Published on: May 13, 2019
In vitro interaction between the N-terminus of the Ewing's sarcoma protein and the subunit of RNA polymerase II
1Institute of Biophysics, Bulgarian Academy of Sciences, Acad. Georgi Bonchev str., Bl. 21, 1113, Sofia, Bulgaria. todorova@obzor.bio21.bas.bg
Abstract:
In vivo and in vitro expressed N-terminal sequence of EWS (EAD) and hsRPB7 (subunit of human RNA polymerase II) were probed for protein-protein interactions using pull-down assays. In result, it was found that the proteins 57Z (residues 1-57 of EAD) and hsRPB7 interact in vitro forming a stable complex. The direct interaction between 57z and hsRPB7 indicate that DHR-related peptides and other small molecules, targeted to N-terminus of EWS might possess therapeutic potentialities as anti-cancer agents to function as inhibitors of EAD-mediated transactivation.
Related Concept Videos
Eukaryotic RNA Polymerases
All three eukaryotic RNAPs require specific transcription factors, of which the...
Eukaryotic RNA Polymerases
All three eukaryotic RNAPs require specific transcription factors, of which the...
Transcription Initiation
The promoters and enhancers and their accessory proteins allow tight regulation of...
RNA Polymerase II Accessory Proteins
RNA Polymerase II Accessory Proteins
Bacterial Transcription
Transcription can be divided into three main stages, each involving distinct DNA sequences to guide the polymerase. These are:

