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Muscle cell attachment in Caenorhabditis elegans.

R Francis¹, R H Waterston

¹Department of Genetics, Washington University School of Medicine, St. Louis, Missouri 63110.

The Journal of Cell Biology

|August 1, 1991

Summary

Researchers identified key molecules anchoring nematode muscles to their outer cuticle. These findings in Caenorhabditis elegans shed light on the fibrous organelle and basement membrane components essential for locomotion.

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Area of Science:

Cell biology
Biochemistry
Developmental biology

Background:

Locomotion in Caenorhabditis elegans relies on body wall muscles exerting force on the cuticle.
A basement membrane and hypodermal cell mediate force transmission between muscle and cuticle.
The hypodermal cell contains a fibrous organelle with filament bundles attached to membrane plaques.

Purpose of the Study:

To define the molecular chain anchoring muscle cells to the cuticle.
To identify components of the basement membrane and fibrous organelle.

Main Methods:

Isolation of five monoclonal antibodies (mAbs) using enriched preparations.
Characterization of antigens recognized by the mAbs.

Main Results:

Two mAbs identified a 200-kD muscle antigen at the muscle/hypodermal basement membrane.
Three mAbs recognized elements of the hypodermal fibrous organelle.
mAb IFA, reactive with mammalian intermediate filaments, also bound these structures.

Conclusions:

Identified potential molecular components involved in muscle-to-cuticle anchoring.
Suggests these components are crucial for tension transmission during locomotion.
Provides insights into the structure and function of the nematode fibrous organelle.

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