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Updated: Jun 10, 2026

Detection of Invasive Pulmonary Aspergillosis in Haematological Malignancy Patients by using Lateral-flow Technology

Detection of Invasive Pulmonary Aspergillosis in Haematological Malignancy Patients by using Lateral-flow Technology

Published on: March 22, 2012

Molecular diagnostic platforms for detecting Aspergillus.

David S Perlin¹, Yanan Zhao

¹Public Health Research Institute, Department of Microbiology and Molecular Genetics, UMDNJ-New Jersey Medical School, Newark, New Jersey 07103, USA. perlin@phri.org

Medical Mycology

|July 9, 2008

Summary

This summary is machine-generated.

New nucleic acid (NA)-based amplification assays offer improved detection of Aspergillus species in invasive aspergillosis. These advanced molecular platforms enhance diagnostic accuracy for fungal infections.

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Area of Science:

Medical Mycology
Molecular Diagnostics
Infectious Diseases

Background:

Invasive aspergillosis diagnosis is challenging, with current methods like imaging and serology having limitations.
Nucleic acid (NA)-based amplification assays were developed to improve pathogen identification but have shown variable success for Aspergillus spp.

Purpose of the Study:

To review emerging nucleic acid platforms for enhanced detection of Aspergillus-specific DNA and RNA.
To highlight the potential of advanced molecular diagnostics in improving the diagnosis and management of invasive aspergillosis.

Main Methods:

Discussion of polymerase chain reaction (PCR) platforms, particularly real-time PCR with multiplexing capabilities.
Overview of nucleic acid sequence-based amplification (NASBA) as a robust RNA-directed isothermal amplification method.
Exploration of allele-specific probe properties for drug resistance marker assessment.

Main Results:

New NA platforms demonstrate high sensitivity, detecting single genomes of Aspergillus spp. from respiratory and blood samples.
Pan-fungal and pan-Aspergillus probes enhance the scope of detection.
RNA-based detection offers additional targets and viability assessment.

Conclusions:

Emerging molecular diagnostic platforms show significant promise for accurate and sensitive detection of Aspergillus species.
These advanced NA assays may expand to include early therapeutic monitoring for invasive aspergillosis.