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Selective liquid chromatographic assay for propylthiouracil in plasma
G R Cannell1, J P Williams, A S Yap
1Conjoint Internal Medicine Laboratory, Royal Brisbane Hospital, Queensland, Australia.
Journal of Chromatography
|March 8, 1991
Summary
A new liquid chromatography method accurately measures propylthiouracil in plasma. This assay uses a specific internal standard and extraction technique to ensure reliable quantification for clinical use.
Area of Science:
- Analytical Chemistry
- Pharmacology
Background:
- Accurate quantification of propylthiouracil (PTU) in biological samples is crucial for therapeutic drug monitoring.
- Existing methods may face interference from co-eluting compounds or lack sensitivity.
Purpose of the Study:
- To develop and validate a sensitive and specific liquid chromatographic assay for quantifying propylthiouracil in human plasma.
Main Methods:
- Development of a liquid chromatographic assay utilizing an internal standard (5-propyl-2-thiouracil).
- Employing a base-treated serum extraction method to remove interfering caffeine.
- Chloroform extraction from plasma for drug isolation.
Main Results:
- The assay demonstrated high specificity, with caffeine effectively removed and no other interfering compounds detected.
- Achieved a recovery rate of 59.4% for propylthiouracil extraction.
- Exhibited excellent precision with intra-assay (5.7%) and inter-assay (3.3%) coefficients of variation.
- Demonstrated linearity up to 3 µg/ml with a lower detection limit of 40 ng/ml for a 1 ml sample volume.
Conclusions:
- The developed liquid chromatographic assay is a reliable and sensitive method for propylthiouracil quantification in plasma.
- This assay provides a valuable tool for clinical pharmacokinetic studies and therapeutic drug monitoring of propylthiouracil.