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Cell retention culture with an internal filter module: continuous ethanol fermentation.

H N Chang¹, W G Lee, B S Kim

¹Department of Chemical Engineering and Bioprocess Engineering Research Center, Korea Advanced Institute of Science and Technology, Taejon 305-701, South Korea.

Biotechnology and Bioengineering

|March 15, 1993

Summary

A novel internal filter system effectively separates yeast for high-density ethanol fermentation. This stainless steel filter technology offers scalable, continuous cell culture comparable to external systems.

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Area of Science:

Biotechnology
Chemical Engineering
Fermentation Technology

Background:

Continuous ethanol fermentation requires efficient cell separation and retention.
External membrane systems are common but can be complex to scale.
High-density cell cultures are crucial for optimizing fermentation productivity.

Purpose of the Study:

To introduce and evaluate a new internal filter feedback system for continuous ethanol fermentation.
To assess the filter's performance in yeast separation and high-density cell culture.
To compare the scalability and operability of the internal filter system with external methods.

Main Methods:

Implementation of a stainless steel internal filter with a 2 µm pore size.
Investigation of filter performance based on agitation speed and yeast concentration.
Operation of the fermentor by manipulating dilution rate and bleed ratio to control cell concentration.
Continuous operation for over 10 days to assess system stability.

Main Results:

The internal filter achieved a retention coefficient greater than 97.5%, proving effective for yeast separation.
Optimal operable yeast concentrations ranged from 90 to 150 g/L, with a maximum observed at 157 g/L.
The system demonstrated stable operation for extended periods, maintaining desired cell densities.
Performance was comparable to external membrane cell recycle cultures.

Conclusions:

The internal filter feedback system is suitable for high-density cell culture and continuous ethanol fermentation.
The stainless steel filter offers a robust and efficient method for yeast separation.
This internal system presents a more easily scalable alternative to external cell recycle systems.